DNA Extraction and Purification
Zymo Research Corporation ZymoPURE II™ Plasmid Maxiprep (20 preps)
ZymoPURE™ Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA from E. coli. The ZymoPURE™ Plasmid Maxiprep kit features a spin column-based method for the purification of up to 3.0 mg of high-quality plasmid DNA in less than 18 minutes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 g/l) directly from a spin column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA resulting in plasmid DNA suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing.
Zymo Research Corporation DNA Clean & Concentrator™-5 (50 Preps) w/ Zymo-Spin™ IC Columns (Capped)
It can also be used for post-RT cDNA clean-up andpurification of sequencing-ready DNA from M13 phage. Simply add the specially formulated DNA Binding Buffer to your sample and transfer the mixture to the supplied Zymo-Spin™ Column. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin column technology to yield DNA that is free of salts and contaminants in just 2 minutes. The purified DNA is ideal for DNA ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures.
Edge Biosystems Inc Performa® DTR Gel Filtration Cartridges 108 pack
The gold standard in gel filtration based dye terminator removal!Performa® DTR utilizes a fully hydrated gel filtration matrix that provides for a convenient and simple method of dye terminator removal for sequencing cleanup. Performa DTR ensures removal of BigDye® Terminator v3.1 or other dye terminators, along with dNTPs, salts and other low molecular weight materials from sequencing reactions. DNA primers and fragments up to 20 bases, buffers, nucleotides labeled with biotin, isotopes and other assorted markers are also eliminated. To minimize the potential for interference with sequencing applications, no preservatives, salts, or buffers are used in the preparation of Performa DTR.
LGC GENOMICS LLC (Lucigen) QuickExtract DNA Extraction Solution 1.0, 50 mL
The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample.
Zymo Research Corporation ZR BashingBead Lysis Tubes (0.1 & 0.5 mm), 50 tubes
The state of the art, ultra-high density beads are fracture resistant, chemically inert, and ideal for disrupting tough-to-lyse biological samples. These beads are ideal for tough-to-lyse bacteria, yeast, fungi and algae.
Agilent Technologies D1000 ScreenTape for DNA analysis
For the analysis of DNA from 35 to 1000 bp. Includes 7 ScreenTape devices.
Zymo Research Corporation ZymoPURE II™ Plasmid Maxiprep (10 preps)
The ZymoPURE II Plasmid Maxiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Maxiprep kit can process up to 150 ml of culture in less than 20 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with either a vacuum or a centrifuge instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 g/l), EndoZero, vaccine-grade, and transfection-ready. The ZymoPURE II Plasmid Maxiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Agilent Technologies High Sensitivity D1000 ScreenTape for DNA Analysis
High Sensitivity D1000 ScreenTape. For the high sensitivity analysis of DNA from 35 to 1000 bp. Includes 7 ScreenTape devices.
Zymo Research Corporation ZymoPURE Plasmid MiniPrep Kit (100 Preps)
features a spin column-based method for the purification of up to 100 µg of ultra-pure endotoxin-free plasmid DNA in less than 15 minutes. The unique spin-column design also provides zero buffer retention and a low elution volume. ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl). In addition, the wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA. The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.
Covaris Inc g-TUBE for DNA Shearing, 10/pk
10 individually-wrapped g-TUBES for shearing DNa 6kb to 20 kb and one g-TUBE Prep Statio
New England Biolabs, Inc. Monarch® Spin gDNA Extraction Kit - 50 preps
The Monarch Spin gDNA Extraction Kit is a comprehensive solution for cell lysis, RNA removal, and purification of intact genomic DNA (gDNA) from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, bacteria and yeast can be processed with extra steps to enhance lysis in these tough-to-lyse samples. Monarch products use significantly less plastic in columns and kits compared to leading suppliers. Protocols are also included to enable purification from clinically-relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA. Purified gDNA has high quality metrics, including A260/A280 >1.8 and A260/A230 >2.0, high DIN scores and minimal residual RNA. Purified gDNA is suitable for downstream applications such as end-point PCR, qPCR and library prep for NGS. It typically has a peak size of >50kb, making this kit an excellent choice upstream of long-read sequencing platforms
Agilent Technologies High Sensitivity D1000 Reagents
For the high sensitivity analysis of DNA from 35 to 1000 bp. Includes ladder and sample buffer.
New England Biolabs, Inc. Thermolabile Proteinase K – 30 units
Thermolabile Proteinase K (TLPK) is an engineered, subtilisin-related serine protease that will hydrolyze a broad variety of peptide bonds. It preferentially cleaves the peptide bond at the carboxyl side of aliphatic or aromatic amino acid residues. TLPK can be completely inactivated by incubation at 55°C for 10 minutes, which allows for subsequent enzymatic steps in the same reaction vessel.
Zymo Research Corporation ZymoPURE II™ Plasmid Midiprep (25 preps)
ZymoPURE™ technology uses a modified alkaline lysis method and features novel binding chemistry, which enables the highest yields and concentration of plasmid DNA (up to 3 g/l) directly from a spin-column. Coupling ZymoPURE with the innovative EndoZero™ II Spin-Columns, to eliminate endotoxins, achieves EndoZeroplasmid DNA ( 0.025 EU/g of plasmid DNA), making it suitable for transfection, restriction endonuclease digestion, in vivo studies, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.As an added convenience, the ZymoPURE™ II Plasmid Midiprep Kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Syringe filters are included for rapid clearing of the lysate and the unique spin-column design allows the binding step to be performed using a vacuum or table top centrifuge.
Macherey-Nagel NucleoSpin Gel and PCR Clean-up, 50 preps
NucleoSpin Gel and PCR Clean-up (50) 50 preps for PCR clean-up and gel extraction - NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes, buffers